Methold for the Accurate Preparation of Cell-Spiking Standards
- Release Time:2025-04-30
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Date:
2025-04-30Title of Paper:
Methold for the Accurate Preparation of Cell-Spiking StandardsJournal:
Analytical ChemistrySummary:
Preparation of calibration standards for cell enumeration is
critical in characterizing the performance of any method or
apparatus intended for recovering rare cells. Diluting a cell
suspension serially is prone to statistical sampling errors
as the cell suspension becomes more dilute, whereas
transferring and injecting cells individually into a diluent
with a micromanipulator is time-consuming. We developed
a simple and robust method using a surface-modified glass
capillary to siphon and eject cells. One-dimensional confinement
of cells offered by the capillary made cell enumeration
by visual counting simple and rapid, and cell ejection from
the capillary was near 100% when the appropriate surface
coating and cell solution was used. The residence time of
cells in the capillary, however, could affect the percentage
of cells that was ejected from the capillary. To characterize
the performance of this method, we enumerated the ejected
cell using both visual counting under a microscope and
automated detection using a chip-based flow cytometer.Co-author:
Yongxi Zhao, Perry G. Schiro, et al. Translation or Not:
NoDate of Publication:
2009-02-19
