课题组合作论文在国际学术期刊eLife发表
点击次数：
发布时间：2024-10-18
发布时间：2024-10-18
文章标题：课题组合作论文在国际学术期刊eLife发表
内容：
近日，国际学术期刊eLife在线发表了王然研究员的合作研究成果Asynchronous mouse embryo polarization leads to heterogeneity in cell fate specification。这项研究揭示了小鼠胚胎在8-细胞阶段的极化过程存在时间上的异步性，部分细胞早期极化而其他细胞则较晚极化。早期极化的细胞更倾向于分化为滋养层，而晚期极化的细胞则可能朝向内细胞团分化。这一发现强调了极化时间对细胞命运决定的重要性，并揭示了极化与胚胎形态发生之间的关系，为理解小鼠胚胎早期发育机制提供了新的视角，同时对人类早期妊娠及相关临床问题具有重要启示。
英文摘要与编辑评价如下：
abstract：
The first lineage allocation in mouse and human embryos separates the inner cell mass (ICM) from the outer trophectoderm (TE). This symmetry breaking event is executed through polarization of cells at the 8-cell stage and subsequent asymmetric divisions, generating polar (TE) and apolar (ICM) cells. Here, we show that embryo polarization is unexpectedly asynchronous. Cells polarizing at the early and late 8-cell stage have distinct molecular and morphological properties that direct their following lineage specification, with early polarizing cells being biased towards producing the TE lineage. More recent studies have also implicated heterogeneities between cells prior to the 8-cell stage in the first lineage allocation: cells exhibiting reduced methyltransferase CARM1 activity at the 4-cell stage are predisposed towards the TE fate. Here, we demonstrate that reduced CARM1 activity and upregulation of its substrate BAF155 promote early polarization and TE specification. These findings provide a link between asymmetries at the 4-cell stage and polarization at the 8-cell stage, mechanisms of the first lineage allocation that had been considered separate.
editor's evaluation:
This important work has substantially advanced our understanding of the molecular basis of symmetry breaking and lineage specification in preimplantation mammalian embryos. The results generated using live imaging are compelling. Quantification of the functional assays is convincing and would be improved by increasing the number of embryos in the evaluations and clearly stating how many embryos are evaluated per experiment.
文章链接如下：
https://doi.org/10.7554/eLife.101140.1.sa2