Ultra-slow voltage-dependent inactivation of the calcium current in guinea-pig and ferret ventricular myocytes
发布时间：2025-04-30
点击次数：
- 发布时间：
- 2025-04-30
- 论文名称：
- Ultra-slow voltage-dependent inactivation of the calcium current in guinea-pig and ferret ventricular myocytes
- 发表刊物：
- Pflugers Arch
- 摘要：
- L-type Ca2+ current, iCa, has been recorded in guinea-pig ventricular myocytes at 36 degrees C using the whole cell patch clamp technique. Intracellular Ca2+ was buffered with ethylenebis(oxonitrilo)tetraacetate (EGTA). An increase in the rate of stimulation from 0.5 to 3 Hz resulted in an abrupt decrease in iCa in the first beat at the high rate, followed by a progressive decrease (tau approx. 7 s) over the next 30 s. The changes were not the result of Ca(2+)-dependent inactivation, because similar changes occurred with either Ba2+ or Na+ as the charge carrier. During 20-s voltage clamp pulses there was an ultra-slow phase of inactivation of Ba2+ or Na+ current through the Ca2+ channel (tau approx. 6 s at 0 mV). This was confirmed by applying test pulses after conditioning pulses of different duration: the Ba2+ current during the test pulse decreased progressively when the duration of the conditioning pulse was increased progressively to 20 s. Ultra-slow inactivation of Ba2+ current was voltage dependent and increased monotonically at more positive potentials. Recovery of Ba2+ current from ultra-slow inactivation occurred with a time constant of 3.7 s at -40 mV and 0.7 s at -80 mV. The gradual decrease in iCa on increasing the rate to 3 Hz may have been the result of the development of ultra-slow voltage-dependent inactivation.
- 合写作者：
- Boyett MR, Honjo H, Harrison SM, Zang WJ, Kirby MS
- 卷号：
- 428(1)
- 页面范围：
- 39-50
- 是否译文：
- 否
- 发表时间：
- 1994-08-16