ATP-dependent desensitization of the muscarinic K+ channel in rat atrial cells
发布时间：2025-04-30
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- 发布时间：
- 2025-04-30
- 论文名称：
- ATP-dependent desensitization of the muscarinic K+ channel in rat atrial cells
- 发表刊物：
- J Physiol
- 摘要：
- 1. Fast desensitization of the muscarinic K+ channel has been studied in excised patches from rat atrial cells. 2. In inside-out patches, ACh was present in the pipette and GTP was applied via the bath to activate the channel. In outside-out patches, GTP was present in the pipette and ACh was applied via the bath to activate the channel. In both cases, during a 30 s exposure to GTP or ACh there was a decline in channel activity as a result of fast desensitization if ATP was present. 3. In inside-out patches, fast desensitization was still observed if the muscarinic ACh receptor was bypassed and the channel was activated by GTP gamma S. This suggests that fast desensitization is a result of a modification of the channel (or the connecting G protein) and not the receptor. 4. In both inside-out and outside-out patches, channel activity was depressed and fast desensitization was reduced or absent, if ATP was not present. 5. The non-hydrolysable analogue of ATP, AMP-PNP, did not substitute for ATP in its effects on the channel. 6. The results are consistent with the hypothesis that fast desensitization of the muscarinic K+ channel is the result of a dephosphorylation of the channel.
- 合写作者：
- Shui Z, Boyett MR, Zang WJ
- 卷号：
- 505 ( Pt 1)
- 页面范围：
- 77-93
- 是否译文：
- 否
- 发表时间：
- 1997-11-15