On-line concentration and determination of all-trans- and 13-cis- retinoic acid in rabbit serum by applying sweeping technique in micellar electrokinetic chromatography
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Date:
2025-04-30Title of Paper:
On-line concentration and determination of all-trans- and 13-cis- retinoic acid in rabbit serum by applying sweeping technique in micellar electrokinetic chromatographyJournal:
Journal of Chromatography ASummary:
A simple and rapid micellar electrokinetic chromatography (MEKC) method with UV detection was developed for the simultaneous separation and determination of all-trans- and 13-cis-retinoic acids in rabbit serum by on-line sweeping concentration technique. The serum sample was simply deproteinized and centrifuged. Various parameters affecting sample enrichment and separation were systematically investigated. Under
optimal conditions, the analytes could be well separated within 17 min, and the relative standard deviations (RSD) of migration times and peak areas were less than 3.4%. Compared with the conventional MEKC injection method, the 18- and 19-fold improvements in sensitivity were achieved, respectively. The proposed method has been successfully applied to the determination of all-trans- and 13-cis-retinoic acids in serum samples from rabbits and could be feasible for the further pharmacokinetics study of all-trans-retinoic acid.Co-author:
YongXi Zhao, Yu Kong et al. Translation or Not:
NoDate of Publication:
2007-11-12
