The promotion function of Berberine for osteogenic differentiation of human periodontal ligament stem cells via ERK-FOS pathway mediated by EGFR
Title of Paper:
The promotion function of Berberine for osteogenic differentiation of human periodontal ligament stem cells via ERK-FOS pathway mediated by EGFR
Summary:
Coptidis Rhizoma binds to the membrane receptors on hPDLSC/CMC, and the active ingredient
Berberine (BER) that can be extracted from it may promote the proliferation and osteogenesis of
periodontal ligament stem cells (hPDLSC). The membrane receptor that binds with BER on the cell
surface of hPDLSC, the mechanism of direct interaction between BER and hPDLSC, and the related
signal pathway are not yet clear. In this research, EGFR was screened as the affinity membrane receptor
between BER and hPDLSC, through retention on CMC, competition with BER and by using a molecular
docking simulation score. At the same time, the MAPK PCR Array was selected to screen the target
genes that changed when hPDLSC was simulated by BER. In conclusion, BER may bind to EGFR on the
cell membrane of hPDLSC so the intracellular ERK signalling pathways activate, and nuclear-related
genes of FOS change, resulting in the effect of osteogenesis on PDLSC.
Co-author:
Jin Liu, Xiaodan Zhao, Dandan Pei, Sicen Wang*, Ang Li*
Papers
