Liquid chromatography coupled with hybrid ion trap time-of-flight mass spectrometry method for the determination of caulophine in rat bio-samples
Title of Paper:
Liquid chromatography coupled with hybrid ion trap time-of-flight mass spectrometry method for the determination of caulophine in rat bio-samples
Summary:
A rapid and precise liquid chromatography coupled with hybrid ion trap/time-of-flight
mass spectrometry method to detect and quantify caulophine and its possible active
metabolites in rat plasma and urine was developed. Samples were prepared by plasma
protein precipitation combined with a liquid-liquid extraction method. The separation
was carried out on an InertSustain® C18 column with a mobile phase comprising
methanol and 0.1% aqueous formic acid solution. The analysiswas complete in 20min
with a flow rate of 0.4 mL/min. Taspine was used as the internal standard. Mass spectrometric
detection was conducted with hybrid ion trap/time-of-flight equipped with
electrospray ionization in the positive ion mode. The calibration curves of caulophine
were linear over the concentration ranges of 0.002–0.20 μg/mL for plasma and 0.005–
0.50 μg/mL for urine with the correlation coefficients greater than 0.998 in both cases.
The method was successfully used to investigate the pharmacokinetics and bioavailability
in rat plasma and urine samples after intragastric and intraperitoneal administration
of caulophine sodium salt.
Co-author:
Dan Lu, Xiao Xu, Chunlei Li, Sicen Wang
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