Xiaofang Hou (Associate Professor )

Pharmaceutical Analysis

食品安全分析小组

1. Xu D, Guo D, Zhang J, Tan X, Deng Z, Hou X*, et al. Innovative tumor interstitial fluid-triggered carbon dot-docetaxel nanoassemblies for targeted drug delivery and imaging of HER2-positive breast cancer. International Journal of Pharmaceutics. 2024;657.
2. Jing Zhang, Dan Xu, Zijie Deng, Xueping Tan, Dongnan Guo, Yanru Qiao, You Li, Xiaofang Hou*, Sicen Wang*, and Junbo Zhang*, Using tungsten oxide quantum-dot enhanced electrochemiluminescence to measure thrombin activity and screen its inhibitors. Talanta, 2024. 267.125267
3. Zijie Deng, Xueping Tan, Dongnan Guo, Jing Zhang, Dan Xu, Xiaofang Hou*, Sicen Wang, Junbo Zhang, Fen Wei, and Dongdong Zhang, MXene-sensitized electrochemiluminescence sensor for thrombin activity detection and inhibitor screening. Microchimica Acta, 2023. 190(8).328
4. Yanru Qiao, Yingdi Shi, Chen Wu, Xiaofang Hou*, Xiaoyan Pan, Zijie Deng, sicen Wang*. Rapid screening and identification of anticoagulation component from carthami flos by two-dimensional thrombin affinity chromatography combined with HPLC-MS/MS. Journal of Separation Science. 2021;44(16):3061-3069.
5. Xiaofang Hou, Meng Sun, Tao Bao, Xiaoyu Xie, Fen Wei, Sicen Wang*. Recent advances in screening active components from natural products based on bioaffinity techniques. Acta Pharmaceutica Sinica B. 2020.10(10): 1800-1813.
6. Yingdi Shi, Wei Sun, Xiaoyan Pan, Xiaofang Hou *, Sicen Wang*, Junbo Zhang. Establishment of thrombin affinity column (TAC)-HPLC-MS/MS method for screening direct thrombin inhibitors from Radix Salviae Miltiorrhiae. Journal of Chromatography B. 2020,1139:121894.
7. Ping Zhang, Yingdi Shi, Xiaoshuang He, Wei Sun, Yanni Lv, Xiaofang Hou *. Study on screening potential allergenic proteins from infant milk powders based on human mast cell membrane chromatography and histamine release assays. Journal of Pharmaceutical Analysis. 2019,9(1):55-61.
8. Dan Xu, Sicen Wang, Xiaofang Hou*, Changshan Sun*. Preliminary Studies on Liquiritin, Deoxyschizandrin, and Tanshinone II A as Potential Anti-Neurodegenerative Disease Agent: Determination by Reverse-Phase Liquid Chromatography in Tianwang Buxin Pills. Journal of Analytical Methods in Chemistry. 2019,2019:1-8.
9. Xiaoshuang He, Ping Zhang, Muhammad Saqib, Xiaofang Hou*, Sicen Wang. Screening active anti-breast cancer compounds from cortex magnolia officinalis by MCF-7 cell membrane chromatography coupled with UHPLC-ESI-MS/MS. Anal. Methods, 2017, 9(33): 4828-4836.
10. Hou, X.; Hu, Y.; Wang, P.; Yang, L.; Al Awak, M. M.; Tang, Y.; Twara, F. K.; Qian, H.; Sun, Y.-P., Modified facile synthesis for quantitatively fluorescent carbon dots. Carbon 2017, 122, 389-394.
11. Hu, Y.; Al Awak, M. M.; Yang, F.; Yan, S.; Xiong, Q.; Wang, P.; Tang, Y.; Yang, L.; LeCroy, G. E.; Hou, X.; Bunker, C. E.; Xu, L.; Tomlinson, N.; Sun, Y. P., Photoexcited State Properties of Carbon Dots from Thermally Induced Functionalization of Carbon Nanoparticles. Journal of materials chemistry. C, Materials for optical and electronic devices 2016, 4 (44), 10554-10561.
12. Wang, P.; Liu, J.-H.; Gao, H.; Hu, Y.; Hou, X.; LeCroy, G. E.; Bunker, C. E.; Liu, Y.; Sun, Y.-P., Host–guest carbon dots as high-performance fluorescence probes. J. Mater. Chem. C 2017, 5 (25), 6328-6335.
13. Meng Sun1, Xiaofang Hou1, Yuanyuan Lin, Jie Zhang and Sicen Wang. An improved cell membrane chromatography method for the simultaneous screening of two epidermal growth factor receptor antagonists from radix scutellariae. Journal of Separation Science. 2015, 38(22):3832-9314  
14.  Xiaofang Hou, MengSun，Xiaoshuang He, Liang Chen, Ping Zhang, Langchong He. Simultaneous quantification of five proteins and seven additives in dairy products with the heart-cutting two-dimensional liquid chromatography method. Journal of Separation Science. 2015, 38(22): 3832-3838.
15.  Xiaofang Hou, Jing Ma, Xiaoshuang He, Liang Chen, Sicen Wang, Langchong He. A stop-flow two-dimensional liquid chromatography method for determination of food additives in yogurt. Analytical Methods. 2015, 7, 2141-2148.
16. Peng Zhang, Xiaofang Hou, Nan Lv, Liuxin Feng, Sicen Wang. Cell Membrane Chromatography with Zonal Elution for Characterization of Seven Alkaloids Binding to α1AAdrenoreceptor. Journal of Liquid Chromatography & Related Technologies 2015, 38, 986-992.
17.  Xiaofang Hou, Sicen Wang, Tao Zhang, Jing Ma, Jie Zhang, Yanmin Zhang, Wen Lu, Huaizhen He, Langchong He*. Recent advances in cell membrane chromatography for traditional Chinese medicines analysis. Journal of Pharmaceutical and Biomedical Analysis. 2014,101: 141-150 
18.  Jing Ma, Bing Zhang, Yunan Wang, Xiaofang Hou*. Comparison of Six Sample Preparation Methods for Analysis of Food Additives in Milk Powder. Food Analytical Methods. 2014,7(6):1345-1352  
19. Jing Ma, Bing Zhang, Yunan Wang, Xiaofang Hou*, Langchong He. Determination of flavor enhancers in milk powder by one-step sample preparation and two-dimensional liquid chromatography. Journal of Separation Science. 2014;37(8):920-926.  
20.  Jing Ma, Xiaofang Hou, Bing Zhang, Yunan Wang, Langchong He. The analysis of carbohydrates in milk powder by a new “heart-cutting” two-dimensional liquid chromatography method. Journal of Pharmaceutical and Biomedical Analysis. 2014 25;91:24-31.
21.  Xiaofang Hou, Xilong Yuan, Bing Zhang, Sicen Wang. Screening active anti‐breast cancer compounds from Cortex Magnolia Officinalisby two dimensional liquid chromatography‐mass spectroscopy. Journal of Separation Science. 2013, 36, 706–712 
22.  Xilong Yuan, Bing Zhang, Yunan Wang, Jing Ma, Xiaofang Hou*. An online coupled breast cancer cell membrane chromatography with HPLC/MS for screening active compounds from Fructus evodiae. Analytical Methods, 2013,5, 5767-5774.   
23. Xiaofang Hou, Sicen Wang, Jingjing Hou, Langchong He. Establishment of A431 Cell Membrane Chromatography-RPLC Method for Screening Target Components from Radix Caulophylli. Journal of Separation Science. 2011, 34, 508–513 
24. Miao Li, Xiaofang Hou, Sicen Wang, Qiang Fu, Langchong He. Applications of HPLC/MS in the analysis of traditional Chinese medicines. Journal of Pharmaceutical Analysis, 2011. 1(2): 81-91.
25.  Xiaofang Hou, Jing Ren, Sicen Wang, Langchong He. Establishment of a High Expression of a1A Adrenergic Receptor Cell Membrane Chromatography-RPLC Method for Screening Target Components from Radix Caulophylli. Chromatographia. 2010, 72: 635-640 
26.  Xiaofang hou, Mingzhe Zhou, Qiao Jiang, Sicen Wang, Langchong He. A Vascular Smooth Muscle Cell Membrane Chromatography-Offline-GC/MS Method for Recognition, Separation and Identification of Active Components from Chinese Traditional Medicines. Journal of Chromatography A. 2009,1216: 7081-7087 
27.  XiaoFang Hou, ZhuJun Zhang, Yan Zhao, Jie Ma. Microdialysis sampling and chemi -luminescence detection for in vivo and real-time study of the lead metabolism in rabbit blood. Microchimica acta. 2007, 159: 223–228 
28. Langchong He, Sicen Wang, Guangde Yang, Yanmin Zhang, Changhe Wang, Bingxiang Yuan, Xiaofang Hou. Progress in cell membrane chromatography. Drug discoveries & therapeutics 2007, 1, 104.
29.  侯晓芳, 王嗣岑, 张涛, 贺浪冲.细胞膜色谱法应用于中药质量控制的思考. 世界科学技术-中医药现代化. 2014,16(3): 526-528.
30. 侯晓芳, 杜晖, 贺晓双, 张平, 王嗣岑. 细胞膜色谱法用于药物与受体相互作用研究进展. 药学进展. 2015, 39(12):882-888.
31. 侯晓芳，章竹君，赵琰，马杰. 流动注射化学发光法测定面粉中的增白剂-过氧化苯甲酰. 陕西师范大学学报（自然科学版）.2006,34(1): 67-70

主持项目

[1]      国家自然科学基金青年基金 （No：81102414） 2012.1-2014.12   23.00万元

“MCF-7/CMC和MDA-MB-231/ CMC法筛选中药中与ER受体相互作用的抗肿瘤活性成分及差异蛋白研究”

[2]      西安交通大学新教师科研支持计划   “吴茱萸中活性成分的乳腺癌细胞膜色谱筛选及提取研究” 2012.1-2014.12  10.00万元

[3]      西安交通大学基本科研业务费学科综合交叉类项目（自然科学类）“乳制品中蛋白质安全评价系统的构建” 2013.1-2015.12  9.00万元

参与项目

[1] 国家自然科学基金 重点项目 （项目编号：70730110）2008.1-2011.12

高表达TKR细胞膜色谱模型的建立与应用基础研究（第十完成人）

[2] 国家自然科学基金（批准号：81073038）  2010.1-2012.12

双通道EGER/CMC法及两种“七药”中抗癌活性成分筛选研究（第二完成人）

[3] 重点实验室青年学术骨干培植项目 （批准号：2010-11）

双通道EGFR/CMC中药筛选方法的建立及中药中抗肿瘤活性成分筛选（第三完成人）

[4] 国家自然科学基金（批准号：30801450） 2009.1-2011.12

α _1A 受体高表达CMC-HPLC/MS联用法筛选红毛七生物碱中活性成分（第三完成人）

[5] 新世纪优秀人才支撑计划（批准号：NCET-08-0437） 2009.1-2011.12

A431 CMC-HPLC/MS联用法筛选发现红毛七中抗肿瘤活性成分 （第六完成人）

[6] 国家自然科学基金 （批准号：30973673） （第五完成人） 2008.1-2010.12

应用PF-2D分析技术筛选乳腺癌化疗耐药分子标志物的实验研究

[7]  国家自然科学基金青年基金（No：81202495）2013.01-2015.12  23.00 万元

      “FGFR4/CMC-HPLC/MS 筛选白芥子促肿瘤细胞凋亡活性成分” （第二完成人）

[8] 国家重大科研仪器设备研制专项（No：81227802）2013.1-2017.12  720.00万元  

      “天然药物中目标物快速“识别鉴定”二维色谱仪研制”  （第七完成人）

[9 ] 国家自然科学基金 重点项目（No：81230079） 2013.1-2017.12  280.00万元

   “细胞膜色谱技术用于中药安全性和有效性评价的基础研究”  （第七完成人）

[1] Jing Zhang, Dan Xu, Zijie Deng, Xueping Tan, Dongnan Guo, Yanru Qiao, You Li, Xiaofang Hou*, Sicen Wang, and Junbo Zhang, Using tungsten oxide quantum-dot enhanced electrochemiluminescence to measure thrombin activity and screen its inhibitors. Talanta, 2024. 267.125267
[2]  Zijie Deng, Xueping Tan, Dongnan Guo, Jing Zhang, Dan Xu, Xiaofang Hou*, Sicen Wang, Junbo Zhang, Fen Wei, and Dongdong Zhang, MXene-sensitized electrochemiluminescence sensor for thrombin activity detection and inhibitor screening. Microchimica Acta, 2023. 190(8).328
[3] Yanru Qiao, Yingdi Shi, Chen Wu, Xiaofang Hou*, Xiaoyan Pan, Zijie Deng, sicen Wang*. Rapid screening and identification of anticoagulation component from carthami flos by two-dimensional thrombin affinity chromatography combined with HPLC-MS/MS. Journal of Separation Science. 2021;44(16):3061-3069.
[4] Xiaofang Hou, Meng Sun, Tao Bao, Xiaoyu Xie, Fen Wei, Sicen Wang*. Recent advances in screening active components from natural products based on bioaffinity techniques. Acta Pharmaceutica Sinica B. 2020.10(10): 1800-1813.
[5] Yingdi Shi, Wei Sun, Xiaoyan Pan, Xiaofang Hou *, Sicen Wang*, Junbo Zhang. Establishment of thrombin affinity column (TAC)-HPLC-MS/MS method for screening direct thrombin inhibitors from Radix Salviae Miltiorrhiae. Journal of Chromatography B. 2020,1139:121894.
[6] Ping Zhang, Yingdi Shi, Xiaoshuang He, Wei Sun, Yanni Lv, Xiaofang Hou *. Study on screening potential allergenic proteins from infant milk powders based on human mast cell membrane chromatography and histamine release assays. Journal of Pharmaceutical Analysis. 2019,9(1):55-61.

[7] Dan Xu, Sicen Wang, Xiaofang Hou*, Changshan Sun*. Preliminary Studies on Liquiritin, Deoxyschizandrin, and Tanshinone II A as Potential Anti-Neurodegenerative Disease Agent: Determination by Reverse-Phase Liquid Chromatography in Tianwang Buxin Pills. Journal of Analytical Methods in Chemistry. 2019,2019:1-8.

[8] 侯晓芳,施迎娣,王嗣岑*. 中药及保健品中非法添加物的分析技术研究进展. 中国药科大学学报. 2019,50(1):33-40

[9]  Xiaoshuang He, Ping Zhang, Muhammad Saqib, Xiaofang Hou*, Sicen Wang. Screening active anti-breast cancer compounds from cortex magnolia officinalis by MCF-7 cell membrane chromatography coupled with UHPLC-ESI-MS/MS. Anal. Methods, 2017, 9(33): 4828-4836.

[10]     Hou, X.; Hu, Y.; Wang, P.; Yang, L.; Al Awak, M. M.; Tang, Y.; Twara, F. K.; Qian, H.; Sun, Y.-P., Modified facile synthesis for quantitatively fluorescent carbon dots. Carbon 2017, 122, 389-394.

[11]     Hu, Y.; Al Awak, M. M.; Yang, F.; Yan, S.; Xiong, Q.; Wang, P.; Tang, Y.; Yang, L.; LeCroy, G. E.; Hou, X.; Bunker, C. E.; Xu, L.; Tomlinson, N.; Sun, Y. P., Photoexcited State Properties of Carbon Dots from Thermally Induced Functionalization of Carbon Nanoparticles. Journal of materials chemistry. C, Materials for optical and electronic devices 2016, 4 (44), 10554-10561.

[12]     Wang, P.; Liu, J.-H.; Gao, H.; Hu, Y.; Hou, X.; LeCroy, G. E.; Bunker, C. E.; Liu, Y.; Sun, Y.-P., Host–guest carbon dots as high-performance fluorescence probes. J. Mater. Chem. C 2017, 5 (25), 6328-6335.

[13]     Meng Sun¹, Xiaofang Hou¹, Yuanyuan Lin, Jie Zhang and Sicen Wang. An improved cell membrane chromatography method for the simultaneous screening of two epidermal growth factor receptor antagonists from radix scutellariae. Journal of Separation Science. 2015, 38(22):3832-9314  IF:2.737

[14]     Xiaofang Hou, MengSun，Xiaoshuang He, Liang Chen, Ping Zhang, Langchong He. Simultaneous quantification of five proteins and seven additives in dairy products with the heart-cutting two-dimensional liquid chromatography method. Journal of Separation Science. 2015, 38(22): 3832-3838.

[15]     Xiaofang Hou, Jing Ma, Xiaoshuang He, Liang Chen, Sicen Wang, Langchong He. A stop-flow two-dimensional liquid chromatography method for determination of food additives in yogurt. Analytical Methods. 2015, 7, 2141-2148.

[16]     Peng Zhang, Xiaofang Hou, Nan Lv, Liuxin Feng, Sicen Wang. Cell Membrane Chromatography with Zonal Elution for Characterization of Seven Alkaloids Binding to α1AAdrenoreceptor. Journal of Liquid Chromatography & Related Technologies 2015, 38, 986-992.

[17]     Xiaofang Hou, Sicen Wang, Tao Zhang, Jing Ma, Jie Zhang, Yanmin Zhang, Wen Lu, Huaizhen He, Langchong He*. Recent advances in cell membrane chromatography for traditional Chinese medicines analysis. Journal of Pharmaceutical and Biomedical Analysis. 2014,101: 141-150  

[18]  Jing Ma, Bing Zhang, Yunan Wang, Xiaofang Hou*. Comparison of Six Sample Preparation Methods for Analysis of Food Additives in Milk Powder. Food Analytical Methods. 2014,7(6):1345-1352  

[19]  Jing Ma, Bing Zhang, Yunan Wang, Xiaofang Hou*, Langchong He. Determination of flavor enhancers in milk powder by one-step sample preparation and two-dimensional liquid chromatography. Journal of Separation Science. 2014;37(8):920-926.  

[20]  Jing Ma, Xiaofang Hou, Bing Zhang, Yunan Wang, Langchong He. The analysis of carbohydrates in milk powder by a new “heart-cutting” two-dimensional liquid chromatography method. Journal of Pharmaceutical and Biomedical Analysis. 2014 25;91:24-31.

[21]  Xiaofang Hou, Xilong Yuan, Bing Zhang, Sicen Wang. Screening active anti‐breast cancer compounds from Cortex Magnolia Officinalis by two dimensional liquid chromatography‐mass spectroscopy. Journal of Separation Science. 2013, 36, 706–712 

[22]  Xilong Yuan, Bing Zhang, Yunan Wang, Jing Ma, Xiaofang Hou*. An online coupled breast cancer cell membrane chromatography with HPLC/MS for screening active compounds from Fructus evodiae. Analytical Methods, 2013,5, 5767-5774. 

[23]  Xiaofang Hou, Sicen Wang, Jingjing Hou, Langchong He. Establishment of A431 Cell Membrane Chromatography-RPLC Method for Screening Target Components from Radix Caulophylli. Journal of Separation Science. 2011, 34, 508–513 

[24]  Miao Li, Xiaofang Hou, Sicen Wang, Qiang Fu, Langchong He. Applications of HPLC/MS in the analysis of traditional Chinese medicines. Journal of Pharmaceutical Analysis, 2011. 1(2): 81-91.

[25]  Xiaofang Hou, Jing Ren, Sicen Wang, Langchong He. Establishment of a High Expression of a _1A Adrenergic Receptor Cell Membrane Chromatography-RPLC Method for Screening Target Components from Radix Caulophylli. Chromatographia. 2010, 72: 635-640 

[26]  Xiaofang hou, Mingzhe Zhou, Qiao Jiang, Sicen Wang, Langchong He. A Vascular Smooth Muscle Cell Membrane Chromatography-Offline-GC/MS Method for Recognition, Separation and Identification of Active Components from Chinese Traditional Medicines. Journal of Chromatography A. 2009,1216: 7081-7087 

[27]  XiaoFang Hou, ZhuJun Zhang, Yan Zhao, Jie Ma. Microdialysis sampling and chemi -luminescence detection for in vivo and real-time study of the lead metabolism in rabbit blood. Microchimica acta. 2007, 159: 223–228 

[28]  Langchong He, Sicen Wang, Guangde Yang, Yanmin Zhang, Changhe Wang, Bingxiang Yuan, Xiaofang Hou. Progress in cell membrane chromatography. Drug discoveries & therapeutics 2007, 1, 104-107.

[29]  侯晓芳, 王嗣岑, 张涛, 贺浪冲.细胞膜色谱法应用于中药质量控制的思考. 世界科学技术-中医药现代化. 2014,16(3): 526-528.

[30]  侯晓芳, 杜晖, 贺晓双, 张平, 王嗣岑. 细胞膜色谱法用于药物与受体相互作用研究进展. 药学进展. 2015, 39(12):882-888.

[31]  侯晓芳，章竹君，赵琰，马杰. 流动注射化学发光法测定面粉中的增白剂-过氧化苯甲酰. 陕西师范大学学报（自然科学版）.2006,34(1): 67-70

2003年-2006年  陕西师范大学  化学与材料工程学院  分析化学专业  

                          研究方向：光学生物传感器    导师：章竹君教授

2006年-2010年  西安交通大学  医学部药学院    药物分析专业 

                          研究方向：细胞膜色谱及二维液相色谱   导师：贺浪冲教授

2016年-2017年  美国Clemson University  化学材料学院   纳米材料专业 

                          研究方向：碳纳米材料       导师：Prof. Ya-Ping Sun

• 2023年-2028年 任中国药学会药物分析专业委员会青年委员会委员

• 2016年-2026年 任陕西省药学会药物分析专业委员会副主任委员兼秘书

• 2017年-2018年 任国家药典委员会理化分析专业委员会秘书

• 2009年-2015年 任中国药学会药物分析专业委员会秘书

地址：中国西部创新港21号楼5133
邮编：710061
QQ：874686566

E-mail：

houxiaofang@mail.xjtu.edu.cn 

侯晓芳  副教授

药物分析学 博士