Date:

2025-04-30

Title of Paper:

Involvement of volume-sensitive chloride channels in the proliferation of human subcutaneous preadipocytes

Journal:

Clin Exp Pharmacol Physiol

Summary:

1. Obesity imposes an important challenge in public health and preventive medicine. Inhibition of preadipocytes proliferation is believed to have an important role in the proposed anti-obesity mechanism. The present study was design to examine the interplay between chloride channels and their possible involvement in the proliferation of undifferentiated human preadipocytes. 2. Preadipocytes are isolated from human abdominal subcutaneous adipose tissue. Membrane ionic currents were recorded using whole-cell patch-clamp technique. The chloride channel gene and protein expression were analyzed by RT-PCR and western blot, respectively. Cell proliferation was evaluated using the [(3)H]-thymidine incorporation assay. 3. Our electrophysiological recording revealed a volume-sensitive chloride current (I(Cl.vol)) expressed in preadipocytes, which was activated by hyposmotic (0.8 T) conditions and inhibited by the chloride channel blocker tamoxifen. 4. Expression of the chloride ion channel gene and protein, ClC-3, was confirmed by RT-PCR and western blot analysis. Blocking I(Cl.vol) with tamoxifen supressed the proliferation of preadipocytes in a concentration-dependent manner. 5. Collectively, our results indicated that the volume-sensitive chloride channel participates in regulating the proliferation of human subcutaneous preadipocytes.

Co-author:

Hu H, Zang WJ*, et al

Translation or Not:

No

Date of Publication:

2009-06-08