Date:

2025-04-30

Title of Paper:

HPLC with column switching coupled to APCI-MS for pharmacokinetic study of amygdalin in rabbit plasma

Journal:

Chromatographia

Summary:

A fast and validated assay was established for the pharmacokinetic study of amygdalin in Armeniacae Semen in rabbit. The method involved column switching (CS) enrichment, separation, post-column derivatization, and atmospheric pressure chemical ionization (APCI) mass spectrometric detection. Plasma sample was enriched by CS using a MAYI-ODS as the first column. Analytes of interest were isolated and analyzed on a second column of Zorbax SB-C18. To detect amygdalin in plasma samples, a T-piece was connected between the HPLC outlet and the APCI source to add a mixture of dichloromethane and methanol to the eluent by an isocratic pump. Calibration graphs showed good linearity over a range of 1.0–1,280 ng mL−1. The detection limit was 0.2 ng mL−1. The intra- and inter-day accuracies were within 3.9%. The method was successfully applied to a study of the pharmacokinetics of amygdalin after an intravenous injection of amygdalin extracts to rabbits with a dose of 400 mg kg−1. The results indicate that amygdalin is a one-compartment open model with a first order absorption phase.

Co-author:

Zhao XF, Zang WJ* et al

Volume:

65

Page Number:

149-153

Translation or Not:

No

Date of Publication:

2006-12-15