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Spectral characters of Rhodamine B as a multi-information fluorescence probe for bovine serum albumins

发布时间:2025-04-30
点击次数:
发布时间:
2025-04-30
论文名称:
Spectral characters of Rhodamine B as a multi-information fluorescence probe for bovine serum albumins
发表刊物:
SENSORS AND ACTUATORS B-CHEMICAL
摘要:
The mechanisms of interaction between bovine serum albumin (BSA) with Rhodamine B (RB) have been
investigated using fluorescence spectroscopy. The quenching of fluorescence from BSA takes place when
increasing the RB concentration. Similar fluorescence quenching fromRB also takes place when increasing
the BSA concentration. Dual quenching is attributed to the binding of RB to BSA and the energy transfer
between BSA and RB. In this interaction, BSA as well as RB acts as both donor and acceptor. Furthermore,
increasing the concentration of RB also leads to BSA fluorescence peak splitting at 345 nm. Therefore, not
only the intensity, but also shape and peak position of the fluorescence spectrum from BSA are controllable
by the concentration of RB. This multi-information fluorescence probe method indicates potential
applications in bio-optical sensor.  © 2009 Elsevier B.V. All rights reserved.
合写作者:
Jian Zhu, Jian-Jun Li, Jun-Wu Zhao
卷号:
138
页面范围:
9-13
是否译文:
发表时间:
2009-04-24