Date:

2025-04-30

Title of Paper:

Specific detection of carcinoembryonic antigen based on fluorescencequenching of Au-Ag core-shell nanotriangle probe

Journal:

Sensors and Actuators B: Chemical

Summary:

Specific detection of carcinoembryonic antigen based on fluorescencequenching of Au-Ag core-shell nanotriangle probe

Gold-silver bimetallic fluorescence quenching nanoprobe has been synthesized based on the gold triangu-lar nanoplate with silver coating. In order to guarantee the specificity of the probe, surface modificationsof the triangular nanoplates including carboxyl replacement, carboxyl activation and antibody connec-tion have been carried out. The fluorescence spectral study indicates that the fluorescence intensityof carcinoembryonic antigen (CEA) could be effectively quenched by the silver coated-gold triangularnanoprobe. And the fluorescence quenching ability could be improved by optimizing the interactiontime and amount of binding anti-CEA to modified nanoprobe, and the interaction time of binding CEAto anti-CEA. By using this sensing method, the CEA could be detected with an ultra low detection limitof 10 pg/mL, and the linear range spans a scope from 20 to 200 pg/mL. Interference experiment resultsshow that the influence from the interfering proteins could be neglected in the detection procedure. Andthe CEA could be specifically detected by using this bimetallic triangular nanoplate probe.© 2016 Elsevier B.V. All rights reserved.

Co-author:

Jian Zhu∗, Jing-Fei Wang, Jian-Jun Li, Jun-Wu Zhao∗

Translation or Not:

No

Date of Publication:

2016-10-05