发布时间：2025-04-30

论文名称：Simultaneous determination of camptothecin and 10-hydroxycamptothecine in the Camptotheca acuminate, its medicinal preparation and in rat plasma by liquid chromatography with fluorescence detection

发表刊物：Biomed Chromatogr

摘要：Camptotheca acuminata Decne is an important medicinal plant that contains various cytotoxic alkaloids, such as camptothecine (CPT) and 10-hydroxycamptothecine (HCPT). A rapid and sensitive liquid chromatography with fluorescence detection (LC-FLD) method for the quantification of CPT and HCPT is described. The separation was carried out on a DL-C l8 column (4.6×150mm, 5μm), with the mobile phase of acetonitrile–sodium dihydrogen phosphate buffer (10m M ) using an gradientelutionattheflowrateof0.6mL/min.TheLC-FLDmethodwasvalidatedforlinearity,sensitivity,accuracyandprecision, and then used to determine the content of the above components. The lower detection limits of CPT and HCPT were 0.4 and 0.1ng/mL, respectively. The precision was <1.58% and the mean recovery of the analytes was 96.0–98.6%. The LC-FLD method was successfully applied to determine CPT and HCPT in real samples including C. acuminate, HCPT injection and rat plasma.

合写作者：Peng Li, Qinhua Chen, Fei Yu, Xiaoyu Xie, Sicen Wang*

卷号：2015, 29

页面范围：1522-1526

是否译文：否

发表时间：2015-01-19