发布时间：2025-04-30

论文名称：Rapid determination of three alkaloids from Lotus Plumule in human serum using an HPLC-DAD method with a short monolithic column

发表刊物：Food Chemistry

摘要：An HPLC-DAD with a short monolithic column method was used for rapid, sensitive and simultaneous determination of liensinine, isoliensinine and neferine in human serum. Chromatographic separation was achieved on an RP-HPLC chromolithic column (50 mm × 4.6 mm, i.d.), with a mobile phase of methanol-water-triethylamine-acetic acid (70:30:0.2:0.05), at a flow rate of 1 ml min-1. The calibration curves of three alkaloids exhibit good linear relationship in the concentration range of 0.5-80 μg ml-1. The limits of detection (LODs) of three alkaloids were 20 ng ml-1, 20 ng ml-1 and 25 ng ml-1, respectively, and the separation time was 4 min. A comparative study has been performed with a normal C18 packed column (150 mm × 4.6 mm i.d., 5 μm). The separation time was 16 min and the LODs of three alkaloids were 56 ng ml-1, 56 ng ml-1 and 65 ng ml-1, respectively.

合写作者：Hui Du, Jing Ren, Sicen Wang*

卷号：2011, 129

页面范围：1320-1324

是否译文：否

发表时间：2011-07-07