发布时间：2025-04-30

论文名称：A combined A431 cell membrane chromatography and online high performance liquid chromatography/mass spectrometry method for screening compounds from total alkaloid of Radix Caulophylli acting on the h

发表刊物：Journal of Chromatography B

摘要：We have developed an online analytical method that combines A431 cell membrane chromatography
(A431/CMC)with high performance liquid chromatography andmass spectrometry (LC/MS) for identify-
ing active components from Radix Caulophylli acting on human EGFR. Retention fractions on A431/CMC
model were captured onto an enrichment column and the components were directly analyzed by com-
bining a 10-port column switcher with an LC/MS system for separation and preliminary identification.
Using Sorafenib tosylate as a positive control, taspine and caulophine from Radix Caulophylli were iden-
tified as the activemolecules which could act on the EGFR. This A431/CMC-online-LC/MSmethod can be
applied for screening active components acting on EGFR fromtraditional Chinesemedicines exemplified
by Radix Caulophylli andwill be of great utility in drug discovery using naturalmedicinal herbs as a source
of novel compounds.

合写作者：M Sun, J Ren, H Du, SC Wang(通讯）, LC He

卷号：878(28)

页面范围：2712-2718

是否译文：否

发表时间：2010-09-08