Simultaneous identification and characterization of amanita toxins using liquid chromatography-photodiode array detection-ion trap and time-offlight mass spectrometry and its applications
Title of Paper:
Simultaneous identification and characterization of amanita toxins using liquid chromatography-photodiode array detection-ion trap and time-offlight mass spectrometry and its applications
Summary:
Rapid and accurate identification of multiple toxins for clinical diagnosis and treatment of mushroom poisoning
cases is still a challenge, especially with the lack of authentic references. In this study, we developed an effective
method for simultaneous identification of amanita peptide toxins by liquid chromatography coupled with
photodiode array detection and ion trap time-of-flight mass spectrometry. The accuracy and selectivity of the
methodology were validated through similar multiple fragmentation patterns and characteristic ions of standard
α- and β-amanitin. The developed method could successfully separate and identify major toxic constituents in
Amanita mushrooms. Two amatoxins and three phallotoxins were confirmed in a single run through their
fragmentation patterns and characteristic ions, which can be used as diagnostic fragment ions to identify
mushroom toxins in complex samples. Furthermore, the performance of the developed method was verified by
using real biological samples, including plasma and urine samples collected from rats after intraperitoneal administration
of toxins. Thus, the development methodology could be crucial for the accurate detection of
mushroom toxins without standard references.
Co-author:
Chunlei Li, Hongyan Qian, Tao Bao, Guangde Yang, Sicen Wang*, Xinshe Liu*
Papers
