Simultaneous determination of camptothecin and 10-hydroxycamptothecine in the Camptotheca acuminate, its medicinal preparation and in rat plasma by liquid chromatography with fluorescence detection
Title of Paper:
Simultaneous determination of camptothecin and 10-hydroxycamptothecine in the Camptotheca acuminate, its medicinal preparation and in rat plasma by liquid chromatography with fluorescence detection
Summary:
Camptotheca acuminata Decne is an important medicinal plant that contains various cytotoxic alkaloids, such as camptothecine (CPT) and 10-hydroxycamptothecine (HCPT). A rapid and sensitive liquid chromatography with fluorescence detection (LC-FLD) method for the quantification of CPT and HCPT is described. The separation was carried out on a DL-C l8 column (4.6×150mm, 5μm), with the mobile phase of acetonitrile–sodium dihydrogen phosphate buffer (10m M ) using an gradientelutionattheflowrateof0.6mL/min.TheLC-FLDmethodwasvalidatedforlinearity,sensitivity,accuracyandprecision, and then used to determine the content of the above components. The lower detection limits of CPT and HCPT were 0.4 and 0.1ng/mL, respectively. The precision was <1.58% and the mean recovery of the analytes was 96.0–98.6%. The LC-FLD method was successfully applied to determine CPT and HCPT in real samples including C. acuminate, HCPT injection and rat plasma.
Co-author:
Peng Li, Qinhua Chen, Fei Yu, Xiaoyu Xie, Sicen Wang*
Papers
