2025-04-30

EGFR cell membrane chromatography online high performance liquid chromatography/mass spectrometry method for screening EGFR Antagonists from Radix Angelicae Pubescentis

Science China B

We have developed a new EGFR cell membrane chromatography (EGFR/CMC)-online-high performance liquid chromatography/mass spectrometry (HPLC/MS) method for screening anti-EGFR antagonists from medicinal herbs such as Radix Angelicae Pubescentis. In this study, the HEK293 EGFR cells with high expression of EGFR were used to prepare cell membrane stationary phase (CMSP) in EGFR/CMC model. The retention fractions on EGFR/CMC model were directly analyzed by combining a 10 port columns switcher with a HPLC/MS system online. As the results, osthole from Radix Angelicae Pubescentis were found to be the active components acted on EGFR like dasatinib as a control drug. There were a good relationship with their inhibiting effects on EGFR secretion and HEK293 EGFR cell growth in vitro. This new EGFR/CMC-online-HPLC/MS method can be applied for screening anti-EGFR antagonists from TCMs for instance Radix Angelicae Pubescentis. It will be a useful method for drug discovery with natural medicinal herbs as a leading compound resource.

SC Wang, M Sun, YM Zhang, J Zhang, LC He

2010,53(11)

2357–2362

No

2010-06-11