Stability designs of cell membrane cloaked magnetic carbon nanotubes for improved life span in drug leads screening
Title of Paper:
Stability designs of cell membrane cloaked magnetic carbon nanotubes for improved life span in drug leads screening
Summary:
Convenient strategies to provide natural cell membranes (CMs)-camouflaged nanomaterials with enhanced stability would prompt the advancement of CMs-coated biomimetic technology and expand the application of these emerging nanomaterials. Herein, we have developed stability-enhanced CMs-camouflaged magnetic carbon nanotubes (MCNTs) to screen drug leads from traditional Chinese medicine (TCMs) that target membrane receptors. By modifying MCNTs with N-ethyl-N′-(3(dimethylamino)propyl) carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS), the resulting covalent immobilized CMs-camouflaged MCNTs have improved stability, where the losing amount (20 mg g−1) was significantly decreased compared with that of the unimmobilized materials (40 mg g−1). The high expression ephrinb2/HEK293 cell lines were used to camouflage the EDC/NHS modified MCNTs (CMCNTs) to endow it with drug-screening sites. Moreover, with inherited properties from CMs, ephrinb2/HEK293 CMs-camouflaged CMCNTs possessed good binding capacity and selectivity, and three potential drug leads as mesaconine, deltaline, and 13dehydroxyindine were screened from Aconitum carmichaeli Debx. The pharmacological assays indicated that mesaconine and 13dehydroxyindine could inhibit cancer cell growth by targeting ephrinb2. As a result, this surface engineering method not only offers an insight into fabrication of stabilized CMs-coated nanomaterials but also inspires more brilliant work in the future and paves the way for the biomimetic functional modification of CNTs for a variety of applications.
Co-author:
Qi Hu, Yusi Bu, Ruiqi Cao, Gao Zhang, Xiaoyu Xie*, Sicen Wang*
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