Mast cell-mediated hypersensitivity to fluoroquinolone is MRGPRX2 dependent.
发布时间：2025-04-30
点击次数：
- 发布时间：
- 2025-04-30
- 论文名称：
- Mast cell-mediated hypersensitivity to fluoroquinolone is MRGPRX2 dependent.
- 发表刊物：
- International Immunopharmacology
- 摘要：
- Fluoroquinolones trigger anaphylaxis during clinical applications, affecting the safety of their administration. Mast cells are immune cells that act as sentinels during host defenses, mediating hypersensitivity and anaphylactic reactions. Mas-related G protein-coupled receptor X2 (MRGPRX2) is a mast cell-specific receptor that mediates cell degranulation in anaphylactic reactions. In this study, the mechanism underpinning the anaphylactic reactions caused by fluoroquinolones was investigated. Hypersensitivity was assessed through hindpaw swelling, tissue fluid leakage assays, in vivo and body temperature measurements assay in vivo, and cell calcium mobilization assays, and mast cell degranulation assays in vitro. Mast cell-deficient W-sash c-kit mutant KitW-sh/W-sh mice and MrgprB2 (the orthologous receptor of MRGPRX2 in mice) knockout mice exhibited reduced fluoroquinolone-induced anaphylactic effects. Fluoroquinolones activated mast cells in a dose-dependent manner and reduced degranulation was observed following MRGPRX2 silencing. These results reveal that fluoroquinolone-induced anaphylactic reactions are mediated by mast cells through MRGPRX2.
- 合写作者：
- Liu R, Hu S, Zhang Y, Che D, Cao J, Wang J, Zhao T, Jia Q, Wang N, Zhang T*.
- 卷号：
- 70
- 页面范围：
- 417-427,
- 是否译文：
- 否
- 发表时间：
- 2019-05-01