Factors influencing the oxygen consumption rate of aortic valve interstitial cells: application to tissue engineering
点击次数：
发布时间：2025-04-30
发布时间：2025-04-30
论文名称：Factors influencing the oxygen consumption rate of aortic valve interstitial cells: application to tissue engineering
发表刊物：Tissue Engineering Part C: Methods
摘要：This study investigated the factors influencing the oxygen metabolism of aortic valve interstitial cells (VICs). Porcine VICs in cell suspension at different passages, and adhered to coverslips at different confluencies, as well as fresh porcine valve leaflets, were incubated in an oxygen respiration chamber at 37°C in Dulbecco's modified Eagle's medium. The consumption rates at different oxygen concentrations were evaluated based on the Michaelis-Menten equation, and the corresponding maximum consumption rate (Vmax) and the Michaelis-Menten equation constant Km were determined. In all cases, the oxygen consumption rate was relatively constant until the concentration dropped to 5% (v/v). The metabolic activity of VICs in terms of oxygen consumption was dependent upon their in vitro passage number and proliferation status. These findings will provide valuable input to the selection of VICs with respect to their age and proliferation status for tissue engineering applications, as well as important input parameters for developing computational models of oxygen transport and optimization of the bioreactor conditions for heart valve tissue engineering.
合写作者：Wang L, Wilshaw SP, Jin Z, et al.
卷号：Volume: 15 Issue 3
页面范围：355-363
是否译文：否
发表时间：2009-08-31