Preparation and endothelialization of multi-level vessel-like network in enzymated gelatin scaffolds
发布时间：2025-04-30
点击次数：
- 发布时间：
- 2025-04-30
- 论文名称：
- Preparation and endothelialization of multi-level vessel-like network in enzymated gelatin scaffolds
- 发表刊物：
- Journal of Bionic Engineering
- 摘要：
- Loss of function of large tissues is an urgent clinical problem. Although the artificial microfluidic network fabricated in large tissue-engineered constructs has great promise, it is still difficult to develop an efficient vessel-like design to meet the requirements of the biomimetic vascular network for tissue engineering applications. In this study, we used a facile approach to fabricate a branched and multi-level vessel-like network in a large muscle scaffolds by combining stereolithography (SL) technology and enzymatic crosslinking mechanism. The morphology of the microchannel cross sections was characterized using micro-computed tomography. The square cross sections were gradually changed to a seamless circular microfluidic network, which is similar to the natural blood vessel. In the different microchannels, the velocity greatly affected the attachment and spread of human umbilical endothelial cell (HUVEC)-green fluorescent protein (GFP). Our study demonstrated that the branched and multi-level microchannel network simulates biomimetic microenvironments to maintain cellular viability. The gelatin scaffolds in the circular vessel-like networks will likely support myoblast and surrounding tissue for clinical use.
- 合写作者：
- GR Dong, Q Lian*, LX Yang, eta
- 是否译文：
- 否
- 发表时间：
- 2018-06-04