Direct-writing Process and in vivo evaluation of prevascularized composite constructs for muscle tissue engineering application
发布时间：2025-04-30
点击次数：
- 发布时间：
- 2025-04-30
- 论文名称：
- Direct-writing Process and in vivo evaluation of prevascularized composite constructs for muscle tissue engineering application
- 发表刊物：
- Journal of Bionic Engineering
- 摘要：
- Tissue engineered skeletal muscle is expected to treat muscle defects caused by trauma and disease. However, designing and manufacturing thick and complex tissue engineered skeletal muscle requires vascularization to ensure its internal cell viability and nutrient supply in vitro. In this article, we developed a set of direct-writing (DW) bioprinting procedure to manufacture a prevascularized composite construct with HUVEC and C2C12 cells for muscle tissue engineering application. We put the cells into the construct in the period of direct writing procedure to obtain the prevascularization and want to promote its vascularization in vivo later. The constructs with cells or without cells were implanted respectively into nude mice back for 3 weeks after which the mice healthily live for all the time and all the implants are tightly bonded to the host. From immunohistochemical analysis, CD31-positive blood vessels existed in the implanted samples with cells are more substantial than those of without cells, but the implanted samples with HUVEC and C2C12 cells had much more number of small blood vessels distributing evenly. Moreover, the implants with cells, especially with HUVEC and C2C12 cells, has been able to get better fusion with the host skin and sub-cutaneous tissues. Histological analysis demonstrates that our direct-writing-based prevascularized constructs have the potential to be getting to vascularize the tissue engineered muscle.
http://link.springer.com/article/10.1007/s42235-020-0037-0
- 合写作者：
- Q Lian*, TZ Zhao, T Jiao, YG Huyan, H Gu, L Gao
- 卷号：
- 17(3)
- 页面范围：
- 457-468
- 是否译文：
- 否
- 发表时间：
- 2020-05-24