- 教授
- 博士生导师
- 硕士生导师
- 入职时间：2015-10-26
- 学历：硕博连读
- 性别：男
- 学位：博士
- 在职信息：在职
- 毕业院校：康涅狄格大学
- 所属院系：计算机科学与技术学院
- 学科：计算机科学与技术
Biochemical and Biophysical Research Communications 457
- 发布时间：2025-04-30
- 论文名称：Biochemical and Biophysical Research Communications 457
- 发表刊物：Biochemical and Biophysical Research Communications 457
- 摘要：In this study, we designed a short hairpin RNA vector-based oligodeoxynucleotide (VB-ODN) carrying transcription factor (TF) consensus sequence which could function as a decoy to block TF activity. Specifically, VB-ODN for Nuclear factor-κB (NF-κB) could inhibit cell viability and decrease downstream gene expression in HEK293 cells without affecting expression of NF-κB itself. The specific binding between VB-ODN produced double-stranded RNA and NF-κB was evidenced by electrophoretic mobility shift assay. Moreover, similar VB-ODNs designed for three other TFs also inhibit their downstream gene expression but not that of themselves. Our study provides a new design of decoy for blocking TF activity.
- 合写作者：Xiao Xiao, Yi Gang, Honghong Wang, Jiayin Wang, Lina Zhao, Li Xu, Zhiguo Liu
- 是否译文：否
- 发表时间：2015-02-21
- 合写作者：Xiao Xiao, Yi Gang, Honghong Wang, Jiayin Wang, Lina Zhao, Li Xu, Zhiguo Liu