Biochemical and Biophysical Research Communications 457

• Date:2025-04-30
• Title of Paper:Biochemical and Biophysical Research Communications 457
• Journal:Biochemical and Biophysical Research Communications 457
• Summary:In this study, we designed a short hairpin RNA vector-based oligodeoxynucleotide (VB-ODN) carrying transcription factor (TF) consensus sequence which could function as a decoy to block TF activity. Specifically, VB-ODN for Nuclear factor-κB (NF-κB) could inhibit cell viability and decrease downstream gene expression in HEK293 cells without affecting expression of NF-κB itself. The specific binding between VB-ODN produced double-stranded RNA and NF-κB was evidenced by electrophoretic mobility shift assay. Moreover, similar VB-ODNs designed for three other TFs also inhibit their downstream gene expression but not that of themselves. Our study provides a new design of decoy for blocking TF activity.
• Co-author:Xiao Xiao, Yi Gang, Honghong Wang, Jiayin Wang, Lina Zhao, Li Xu, Zhiguo Liu
• Translation or Not:No
• Date of Publication:2015-02-21