Sculpting nanoparticle dynamics for singlebacteria-level screening and direct bindingefficiency measurement
发布时间：2025-04-30
点击次数：
- 发布时间：
- 2025-04-30
- 论文名称：
- Sculpting nanoparticle dynamics for singlebacteria-level screening and direct bindingefficiency measurement
- 发表刊物：
- NATURE COMMUNICATIONS
- 摘要：
- Particle trapping and binding in optical potential wells provide a versatile platform for various
biomedical applications. However, implementation systems to study multi-particle contact
interactions in an optical lattice remain rare. By configuring an optofluidic lattice, we
demonstrate the precise control of particle interactions and functions such as controlling
aggregation and multi-hopping. The mean residence time of a single particle is found considerably reduced from 7 s, as predicted by Kramer’s theory, to 0.6 s, owing to the
mechanical interactions among aggregated particles. The optofluidic lattice also enables
single-bacteria-level screening of biological binding agents such as antibodies through
particle-enabled bacteria hopping. The binding efficiency of antibodies could be determined
directly, selectively, quantitatively and efficiently. This work enriches the fundamental
mechanisms of particle kinetics and offers new possibilities for probing and utilising unprecedented biomolecule interactions at single-bacteria level.
- 合写作者：
- Y.Z. Shi, S. Xiong, ...,J.H. Wu, T.N. Chen, ,,,,& A.Q. Liu
- 卷号：
- Vol.9
- 页面范围：
- 815 (11 pages)
- 是否译文：
- 否
- 发表时间：
- 2018-02-28