Date:

2025-04-30

Title of Paper:

Proteomic analysis of differentially expressed proteins in 5-fluorouracil-treated human breast cancer MCF-7 cells

Journal:

Clinical & Translational oncology

Summary:

Abstract
BACKGROUND:
5-Fluorouracil (5-Fu) is a commonly used chemotherapeutic agent in clinical care of breast cancer patients. However, the mechanism of how the 5-Fu works is complex and still largely unknown.
OBJECTIVE:
The objective of this study was to understand the mechanism further and explore the new targets of 5-Fu.
METHODS:
The differentially expressed proteins induced by 5-Fu in human breast cancer MCF-7 cells were identified by proteomic analysis. Four differentially expressed proteins were validated using Western blot and quantitative real-time reverse-transcription polymerase chain reaction analysis for protein and mRNA levels. The effect of 5-Fu on MCF-7 cells was determined by cell viability assay, transmission electron microscopy and flow cytometry analysis.
RESULTS:
5-Fu dose-dependently inhibited cell proliferation with the IC50 value of 98.2 μM. 5-Fu also induced obviously morphological change and apoptosis in MCF-7 cells. Twelve differentially expressed proteins involved in energy metabolism, cytoskeleton, cellular signal transduction and tumor invasion and metastasis were identified.
CONCLUSION:
These results may provide a new insight into the molecular mechanism of 5-Fu in therapy of breast cancer.

Co-author:

Jiangxia Cai, Siying Chen, Yalin Dong*

Translation or Not:

No

Date of Publication:

2014-07-01